Journal: Current Issues in Molecular Biology

Article Title: Anti-Inflammatory Response of New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-like HaCaT Keratinocytes

doi: 10.3390/cimb46060364

Figure Lengend Snippet: Effect of MB-2006 and other strains on cell viability. The HaCaT cells were treated with MB-2006 (1 × 10 7 , 1 × 10 8 and 1 × 10 9 cells/mL of culture supernatant), and LAC and LRH (1 × 10 7 , 1 × 10 8 and 1 × 10 9 cells/mL of PBS). The data are presented as the mean ± SEM (n = 3).

Article Snippet: The HaCaT cells were seeded in 96-well plates at a density of 1 × 10 5 cells/well and incubated at 37 °C for 24 h. Then, the cultured HaCaT cells were treated with MB-2006 and other postbiotics (LAC and LRH) at concentrations of 1 × 10 7 , 1 × 10 8 and 1 × 10 9 cells/mL for 1 h. The pretreated HaCaT cells were stimulated with TNF-α (10 ng/mL; R&D Systems, Neapolis, MN, USA) and IFN-γ (10 ng/mL; R&D Systems, Minneapolis, MN, USA) to induce an AD-like physiological state.

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Anti-Inflammatory Response of New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-like HaCaT Keratinocytes

doi: 10.3390/cimb46060364

Figure Lengend Snippet: MB-2006 and other strains affect the expression of cytokines in TNF-α/IFN-γ-induced HaCaT keratinocytes. Evolution of cytokines in concentration at 1 × 10 7 bacteria/mL ( ), 1 × 10 8 bacteria/mL ( ) and 1 × 10 9 bacteria/mL (■). ( A ) Interleukin (IL)-4, ( B ) IL-13, ( C ) thymic stromal lymphopoietin(TSLP), ( D ) IL-31, and ( E ) IL-10. The bars indicate the mean ± SD, and significant differences are shown in comparison to T + I (TNF-α/IFN-γ only treated group). # p < 0.001 vs. the negative control; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. T + I.

Article Snippet: The HaCaT cells were seeded in 96-well plates at a density of 1 × 10 5 cells/well and incubated at 37 °C for 24 h. Then, the cultured HaCaT cells were treated with MB-2006 and other postbiotics (LAC and LRH) at concentrations of 1 × 10 7 , 1 × 10 8 and 1 × 10 9 cells/mL for 1 h. The pretreated HaCaT cells were stimulated with TNF-α (10 ng/mL; R&D Systems, Neapolis, MN, USA) and IFN-γ (10 ng/mL; R&D Systems, Minneapolis, MN, USA) to induce an AD-like physiological state.

Techniques: Expressing, Concentration Assay, Bacteria, Comparison, Negative Control

Journal: Current Issues in Molecular Biology

Article Title: Anti-Inflammatory Response of New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-like HaCaT Keratinocytes

doi: 10.3390/cimb46060364

Figure Lengend Snippet: MB-2006 and other strains affect the expression of chemokines in TNF-α/IFN-γ-induced HaCaT keratinocytes. Evolution of chemokines (TARC, and MDC) in concentration at 1 × 10 7 bacteria/mL ( ), 1 × 10 8 bacteria/mL ( ) and 1 × 10 9 bacteria/mL (■). ( A ) Thymus and activation-regulated chemokine(TARC), and ( B ) Macrophage-derived chemokine(MDC). The bars indicate the mean ± SD, and significant differences are shown in comparison to T + I (TNF-α/IFN-γ only treated group). # p < 0.001 vs. the negative control; *** p < 0.001 vs. T + I.

Article Snippet: The HaCaT cells were seeded in 96-well plates at a density of 1 × 10 5 cells/well and incubated at 37 °C for 24 h. Then, the cultured HaCaT cells were treated with MB-2006 and other postbiotics (LAC and LRH) at concentrations of 1 × 10 7 , 1 × 10 8 and 1 × 10 9 cells/mL for 1 h. The pretreated HaCaT cells were stimulated with TNF-α (10 ng/mL; R&D Systems, Neapolis, MN, USA) and IFN-γ (10 ng/mL; R&D Systems, Minneapolis, MN, USA) to induce an AD-like physiological state.

Techniques: Expressing, Concentration Assay, Bacteria, Activation Assay, Derivative Assay, Comparison, Negative Control

Journal: Current Issues in Molecular Biology

Article Title: Anti-Inflammatory Response of New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-like HaCaT Keratinocytes

doi: 10.3390/cimb46060364

Figure Lengend Snippet: Effect of MB-2006 and other strains on TNF-α/IFN-γ-induced NF-κB activation in HaCaT cells. The protein expression of NF-κB ( A ) was normalized to non-phosphorylated protein. The bars ( B ) indicate the mean ± SD, and significant differences are shown in comparison to T + I (TNF-α/IFN-γ only treated group). # p < 0.001 vs. the negative control; * p < 0.05, *** p < 0.001 vs. T + I.

Article Snippet: The HaCaT cells were seeded in 96-well plates at a density of 1 × 10 5 cells/well and incubated at 37 °C for 24 h. Then, the cultured HaCaT cells were treated with MB-2006 and other postbiotics (LAC and LRH) at concentrations of 1 × 10 7 , 1 × 10 8 and 1 × 10 9 cells/mL for 1 h. The pretreated HaCaT cells were stimulated with TNF-α (10 ng/mL; R&D Systems, Neapolis, MN, USA) and IFN-γ (10 ng/mL; R&D Systems, Minneapolis, MN, USA) to induce an AD-like physiological state.

Techniques: Activation Assay, Expressing, Comparison, Negative Control